In vitro regenerates of sambong (Blumea balsamifera Linn.)
2009
Soriano, T.L. | Cangao, E.C., Department of Agriculture-Bureau of Plant industry, San Andres, Malate, Manila (Philippines)
A procedure for the in vitro culture of sambong (Blumea balsamifera Linn.) which utilizes terminal shoot was developed. Shoot tips cultured in Murashige and Skoog (MS) medium containing 1.0 mg/L benzyl adenine (BA) initiated and regenerated shoot cultures. Roots were formed in shoots grown in Murashige and Skoog medium supplemented with 0.5-1.0 mg/L napthalene acetic acid (NAA). Exposure to high humidity for the first 2 wk and equal proportions of sterile sand, compost and coir dust as potting mix favored the development of sambong plants. Aside from in vitro culture, very young shoot cuttings from in vitro-derived sambong plants could be used for propagation. Furthermore, the mineral content (ash, crude fat, calcium, magnesium and iron) of sambong leaves derived from tissue cultures (in vitro) and from the field (in vivo) were not significantly different from one another. However, in vitro-derived sambong leaves contained significantly greater amount of crude protein and significantly lower amount of carbohydrates and phosphorus than in vivo-derive leaves.
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