Effect of wheat bran concentration on xylanase biosynthesis by Aspergillus niger
2009
Ahmad, Z. (University of Agriculture, Faisalabad (Pakistan). National Inst. of Food Science and Technology) | Butt, M.S. (University of Agriculture, Faisalabad (Pakistan). Dept. of Biochemistry) | Anjum, F.M. (University of Agriculture, Faisalabad (Pakistan). Dept. of Biochemistry) | Asgher, M. (University of Agriculture, Faisalabad (Pakistan). Dept. of Biochemistry)
Wheat bran an indigenous carbon source was tested as substrate by Aspergillus niger for optimum synthesis of xylanase using the submerged fermentation technique. The trials for xylanase production were conducted at three concentration levels (2.5, 3.0 & 3.5%) of wheat bran, four different fermentation temperatures (i.e., 25.0, 27.5, 30.0 & 32.5 degree C) and four various initial pH levels (5.0, 5.5, 6.0 & 6.5) of the culture medium for a period of 96 h. It is deduced from the study, that the organism exhibited maximum enzyme activity (78.03 plus minus 2.73 IU/mL) at 3.0% wheat bran concentration followed by 66.07 plus minus 1.94 and 64.47 plus minus 2.75 IU/mL at 3.5 and 2.5% concentration, respectively at 30 degree C, 5.5 pH after a period 72 h of incubation. The comparison of the effect of various pH levels of culture medium exhibited that pH 5.5 had enhanced role in xylanase synthesis as compared to other pH levels investigated in the study. Time scale analysis revealed that a fermentation period of 72 h was best suitable for obtaining maximum yields of xylanase. Moreover a temperature of 30 degree C was found to be optimum for higher yields of xylanase exhibiting the mesophillic behavior of the organism.
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