Analysis of endosperm proteins and genetic diversity in Pakistani wheat varieties
2010
Nisar, M.F. (Gujrat Univ. (Pakistan). Dept. of Botany) | Hussain, K. (Gujrat Univ. (Pakistan). Dept. of Botany) | Majeed, A. (Gujrat Univ. (Pakistan). Dept. of Botany) | Nawaz, K. (Gujrat Univ. (Pakistan). Dept. of Botany)
The present study was undertaken to evaluate the genetic diversity in gluten subunits and protein content in seed endosperm of wheat for nutritional grading of 60 registered Pakistani wheat varieties using SDS-PAGE and this grading is useful for agricultural development regarding food stuffs and breeding purposes. After calculating the standard factor in spectrophotometer for unit absorbance at 540 nm and the amount of protein were calculated. The wheat varieties GA-2002, C-250 and Chakwal-86 contains maximum amount of HMW-Gs proteins as compared to rest of the varieties. By comparing with the thirteen bands of the standard marker band numbers 5, 9, 12 and 13 were common in all sixty wheat varieties but the other bands show variation. Most frequent HMW protein bands were with mol. Wt. 110 KDa marker band, while in LMW protein bands most frequent bands were among the 14 KDa marker band. In cluster analysis the above mentioned three wheat variet ies grouped in the same cluster showing that they originate from the same parental line and genetically less diverged. However, the only reason for producing variable amounts of HMW-Gs was due to the varying combinations and expression of genes in these lines.
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