Effect of growth hormones on micropropagation of Vitis vinifera L. cv. Perlette
2008
Jaskani, M.J. (University of Agriculture, Faisalabad (Pakistan). Inst. of Horticultural Sciences) | Abbas, H. (Karachi Univ. (Pakistan). Dr. A.Q. Khan Inst. of Biotechnology and Genetic Engineering) | Sultana, R. (University of Agriculture, Faisalabad (Pakistan). Inst. of Horticultural Sciences) | Khan, M.M. | Qasi, M. | Khan, I.A.
In vitro propagation of Vitis offers opportunities for increasing plant material for cultivation. Cultures were established and maintained in vitro on MS medium supplemented with BA (0, 5 and 10 muM) for shoot and NAA (0, 1, 5 and 10 muM) for callus induction. The best explant sterilization was achieved with 10% chlorox treatment either for 10 or 15 minutes. Oxidative browning was effectively controlled by three subcultures of explants on fresh media. Better shooting was observed on MS medium with 5 muM BA. Shoot proliferation (80%) was obtained by subculturing the micro cuttings on same media as used for shoot formation. Maximum rooting (80%) occurred on medium with 10 muM IBA. The highest callus induction was 80% from stem segments on 5 muM BA followed by leaf disk explants on NAA (1 muM). All callus cultures initiated aerial roots. Leaf disk explants (60%) developed embryoids on MS medium supplemented with NAA (2 mg/l).
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