Caracterización molecular de variedades autóctonas españolas de albaricoquero (Prunus armeniaca, L.) a partir de material antiguo
2009
Martín Ramos, C.
In this work we carried out molecular analyses using sixty year old fruits maintained at the Estación Experimental de Aula Dei (EEAD) (Zaragoza) in order to evaluate genetic erosion in apricot and to decide on future germplasm collections. In the last decades, DNA studies using old biological samples have generated an increasing interest due to the good results obtained in the amplification and sequencing of diverse ancient plant and animal tissues. DNA extraction from plant tissues is a critical step in molecular biology protocols. Most of those protocols are laborious and they are often limited by the quantity and quality of the plant material, especially with ancient DNA. Thus, the first objective of this work was to set up an improved DNA extraction protocol from endocarp and testa tissues obtained from old apricot fruits. Second, the maternal origin of those two tissues was checked through molecular analyses to predict the original genotypes from which the fruits were collected. Third, the protocols developed were used to successfully amplify and characterize DNA obtained from ancient apricot fruit samples. Finally, the old apricot genotypes were compared with current genotypes conserved ex situ at germoplasm collections of the Centro de Investigación y Tecnología Agroalimentaria (CITA) and the EEAD. The results obtained allowed to analyze the variability of the old varieties compared to that of the currently conserved varieties. The results will be highly useful to help us to prioritize which apricot varieties should be collected in the future. The approach used in this work can be of great interest to study the genetic erosion that has taken place in apricot and it could be applied to other plant species
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