Examination of the capabilities of attachment and biofilm formation of different Listeria monocytogenes strains
2009
Milanov, D., Scientific Veterinary Institute Novi Sad, Novi Sad (Serbia) | Asanin, R., Faculty of Veterinary Medicine, Belgrade (Serbia) | Vidic, B., Scientific Veterinary Institute Novi Sad, Novi Sad (Serbia) | Katic, V., Scientific Veterinary Institute Novi Sad, Novi Sad (Serbia) | Plavsa, N., Scientific Veterinary Institute Novi Sad, Novi Sad (Serbia)
The objective of this work was to examine the capabilities of 14 Listeria monocytogenes strains of different origin to attach and to form biofilm on glass surface. The strains originating from animals (8 strains), food (4 strains) and feed (1 strain). The referent strain was a human isolate (ATCC 19115). The number of L. monocytogens was determined after three hours of attachment at 5 deg C, 25 deg C and 37 deg C and after 48-hour incubation in tryptone soy broth by the use of standard count technique on blood agar from tenfold dilution. Three days old biofilm of the selected L. monocytogenes strains was examined by scanning microscopy (SEM) and treated with disinfectants for 5 abd 10 minutes. For all the examined L. monocytogenes strains the number of attached bacteria to glass slides for 3 hours of incubation in static conditions at 25 deg C and 37 deg C ranged from 10**2-10**4 cfu/square cm. After three hours of incubation at 5 deg C bacteria did not attach to glass slides. After 48 h of incubation the number of cells that grow on glass slides did not depend on initial attachment and for all the strains it was 10**5-10**7 cfu/square cm. Scanning electron microscopy confirmed the ability of tested strains to sttach on glass surface. L. monocytogenes cells attached to glass surface expressed higher resistance to disinfectants that the cells in suspension.
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