Detection of equine arteritis virus by real-time Taqman(R)reverse transcription - PCR assay
2009
Chenchev, I. | Polychronova, L. | Chakarova, S. | Genova, I., National Diagnostic and Research Veterinary Medical Institute, Sofia (Bulgaria)
The aim of this study is to develop and approve the real time - PCR method to detect the arteritis virus in the nasal swabs, semen plasma and whole blood samples from horses with clinical signs of the disease or estimating the positive antibodies against viral arteritis. In conclusions: 1) In the results of our investigations we developed, optimized and applied the one step and two steps Real Time PCR for detection of Equine Arteritis Virus in semen fluid, whole blood and other biological material; 2) The methods has need of additionally optimizing on its working condition and the good results we supposed using of one step Real Time PCR.
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