Determination of bean common mosaic virus found in Vojvodina region [Serbia] | Determinacija virusa obicnog mozaika pasulja u Vojvodini [Srbija]
2007
Petrovic, D., Institut za ratarstvo i povrtarstvo, Novi Sad (Serbia) | Bagi, F., Poljoprivredni fakultet, Novi Sad (Serbia) | Milosevic, M., Ministarstvo poljoprivrede, sumarstva i vodoprivrede Republike Srbije, Beograd (Serbia) | Vasic, M., Institut za ratarstvo i povrtarstvo, Novi Sad (Serbia) | Ignjatov, M., Institut za ratarstvo i povrtarstvo, Novi Sad (Serbia) | Vujakovic, M., Institut za ratarstvo i povrtarstvo, Novi Sad (Serbia) | Nikolic, Z., Institut za ratarstvo i povrtarstvo, Novi Sad (Serbia)
Our investigation was aimed towards determination of the presence and distribution of economically the most harmful bean virus (Bean common mosaic virus) in Vojvodina during 2006. Some 262 samples from Vojvodina grouped according to symptom types (A, B, C, D) were collected during 2006. Viruses were identified using DAS ELISA test according to the instruction given by antiserum producer (LOEWE Biochemica GmbH, Germany). On the basis of the obtained results it was concluded that BCMV virus was found in 35% of tested plants. The greatest plant infection was found in Conoplja (66%), and the most common symptom type was B. Chosen bean leaf samples were tested using method of reverse transcription and polymerase chain reaction. Four sets of BCMV specific primers were used for each sample testing. Determination of BCMV virus as a member of Potyviridae family was comfirmed by PCR reaction, and by obtaining specific fragments of certain basic pairs. Set of primers specific for Russian and NL-3D strains were used to prove that determined viruses did not belong to the above mentioned strains.
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