Состав компонентов эфирного масла генетически трансформированных корней руты душистой
2009
Kuzovkina, I.N., Russian Academy of Sciences, Moscow (Russian Federation). The K.A. Timiryazev Institute of Plant Physiology | Sarka, S. | Kheteli, E. | Lemberkovich, E. | Seke, E., The Semmelweis University, Budapest (Hungary)
The composition of essential oil obtained by hydrodistillation from genetically transformed roots of common rue (Ruta graveolens L.) was analyzed. It was established by the methods of gas chromatography and complex gas chromatography-mass spectrometry that the major component of rue essential oil was a root-specific sesquiterpene geijerene comprising 67% of total amount of volatile compounds. Furocoumarins (osthole, halepensin and rutacultin) characteristic of intact plant roots were found in essential oil of cultured rue roots. The content of essential oil in genetically transformed rue roots was 0.23% of root dry weight, which is comparable with the one in the roots of intact plants. Long-term maintenance in the in vitro cultured rue roots of a capability for the synthesis of essential oil major components characteristic of intact plants allows to use them for studying the physiological activity of these volatile compounds and their putative role in the plant root interaction in biocoenoses.
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