Proteomic analysis of the fungus Verticillium albo-atrum | Proteomska analiza glive Verticillium albo-atrum
2007
Mandelc, S., Ljubljana Univ. (Slovenia). Biotechnical Fac., Agronomy Dept. | Radisek, S., Cesta Zalskega tabora 2, 3310 Zalec (Slovenia) | Jamnik, P., Ljubljana Univ. (Slovenia). Biotechnical Fac., Agronomy Dept. | Javornik, B.
Verticillium wilt caused by the soilborne phytopathogenic fungus Verticillium albo-atrum is a serious threat to hop production in Slovenia. The fungus invades the plant through its roots and colonizes the vascular tissue, which leads to a disrupted or entirely blocked water supply. Mild and lethal pathotypes are distinguished, depending on the level of virulence. The mild form has been present in Slovenia since 1974 and causes no considerable economic loss, while the lethal form was first discovered in 1997. Due to the lack of effective phytopharmaceutical agents, the only effective means of fighting the disease are phytosanitary and hygienic measures, crop rotation and planting resistant varieties. In order to get a wide insight into the infectious process and to identify proteins related to infection, we employed a proteomic approach, which allows simultanous analysis of total proteins on two-dimensional polyacrylamide gel. The results of comparison of total proteins between the mild and lethal forms are presented.
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