Arpaya dayalı rasyonla beslenen broylerlerde ß-glukanaz geni taşıyan E. coli bakterisinin etkisinin araştırılması

English. The present study was conducted to evaluate the effect of E. coli (DH5α strain) culture producing beta glucanase on growth performance, nutrient utilization and the gut morphology of broilers. One hundred twenty 1-d-old chicks (Cobb 500) were randomly divided into three groups with 5 replicate pens of 8 chicks and assigned to receive corn-based diet (CBD), barley based diet without bacteria (BBD) and barley based diet with addition of bacteria producing beta-glucanase via drinking water (BBDG). Replacing corn with barley in the diets resulted in growth depression despite containing equal amounts of energy and protein (P < 0.01). Birds in the BBDG group consumed more feed, grew faster, and had better feed conversion ratio than those in the group BBD. Inclusion of E. coli decreased the pancreas weight (P < 0.05) but did not affect the liver weight and duedonum, jejunum, ileum, and cecum lengths. Villus length and width, but not crypt width and depth decreased in response to replacing corn with barley in the diets (P< 0.05). However, provision of E. coli strain ameliorated the decreases in villus length and width resulting from barley feeding (P < 0.05). Crypt depth and crypt width were unresponsive to the bacterial treatment. The barley-based diet decreased nutrient digestibility compared with the corn-based diet (P < 0.05). Addition of E. coli strain via water caused improvements in apparent digestibility of dry matter, organic matter, crude protein and ether extract and intestinal glucanase activity (P < 0.05) The present data indicated that supplementation of an E. coli strain capable of producing beta-glucanase with broilers improved nutritive value of barley through altering its digestibility and intestinal morphology.

Turkish. Genetik teknikler kullanılarak β-glukanaz enzim geni aktarılan E. coli bakterisinin barsaklarda yerleşmesi sağlanmış olup bakteri varlığında barsak içerigi glukanaz aktivitesinde, ham besin maddelerinin sindirilme derecelerinde ve broylerlerin performans değerlerinde artış elde edilmiştir.