Genetic polymorphism of alpha S1- casein gene in Bulgarian sheep breeds
2011
Khristova, D., [email protected]
The polymorphism of the CSN1S1 was investigated in three Bulgarian sheep breeds. The study was carried out on total of 90 individuals belonging to Karakachanska, Local Karnobatska and Copper-Red - Mednochervena, Shumenska, that are the most popular local sheep breeds in the country. Two genetic variants of CSN1S1 A and C and three genotypes AA, AC and CC have been identified using PCR-RFLP analysis. The gene frequencies of CSN1S1 A and C were 0,02 and 0,98 in Karakachanska and Copper-Red Shumenska, and 0,10 and 0,90 in Local Karnobatska breeds, respectively. The most common genotype at the αS1-CN locus was CC with frequencies 0,97 in Karakachanska and Copper-Red Shumenska breeds, and 0,84 in Local Karnobatska sheep. These data provide evidence that Bulgarian sheep have a genetic variability, which opens interesting prospects for future selection programs, especially for marker-assisted selection as well as for preservation of the genetic diversity of the populations of these local sheep breeds.
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