Superovulation and embryo transfer in goats by using PMSG or FSH
2011
Pampukidou, A., Agricultural University, Plovdiv (Bulgaria) | Ivanova, R., Agricultural University, Plovdiv (Bulgaria)
The objective of this study was to analyze the efficiency of superovulation, embryo collection, transfer and embryo survival in indigenous goat during breeding season. Twenty-four goats-donors were synchronized by placing an intravaginal sponge of medroxyprogesterone acetate - MAP, 60mg for 17 days. Superovulation was induced in 12 goats by a single injection of PMSG - 1200 IU/animal, i.m., at the time of sponge removal, and in 12 goats by FSH i.m. injections, twice a day, in a total amount of 21mg in reduced consecutive doses, starting at day 15 of the estrus cycle. All donors showing estrus, were bred naturally and 6 days post mating were subjected to a surgical uterine flushing with Dulbeccos phosphate saline enriched with 3% BSA and fresh embryos were transferred surgically to synchronized recipients. Mean interval to onset of estrus did not differ between the two treatments; however the duration of estrus was shorter in FSH - treated group. There was significant difference - p0.05 between treatments in total ovarian response, in ovulation rate - 3.9 ± 0.9 group PMSG, and 12.3 ± 2.0, group FSH and in embryo recovery - 2.3 ± 0.8 for PMSG, and 9.3 ± 1.5 for FSH group. Embryo survival after transfer of embryos was significantly - p0.05 higher for the FSH group - 61.1% and 22.2% for FSH-p, and PMSG.
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