Усовершенствование способа получения ранних зародышей на преимплантационных стадиях вне организма
2009
Gandzha, A.I. | Letkevich, L.L. | Simonenko, V.P. | Kirillova, I.V., National Academy of Sciences. Scientific and Practical Center of Animal Breding (Belarus) | Lobanok, E.S. | Nikolskaya, V.P., National Academy of Sciences, Minsk (Belarus). Institute of Biophysics and Cell Engineering
As a result of researches it was determined that usage of monolayer of somatic cells of follicle and oviduct as a natural source of hormones and factors of growth let us get 17,3-18,9% of embryos at pre-implant stages. Treatment of embryos by direct polarized light after insemination and during the 7 days of cultivation promoted increase of morula-blastocists outcome at up to 25%. It is determined that ATF content in embryo cells depends on functional condition of embryos and makes 3,0-8,0 pmol/k
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