Study on the effect of sijunzi decoction and supplementary sijunzi decoction on immune function in broilers

Chinese. 为开发优质高效的中药免疫增强剂提供参考依据。选用420只1日龄AA肉鸡，随机分成Ⅰ～Ⅶ 7个处理组，每组设3个重复，每重复20只。试验鸡在新城疫Lasota 系冻干苗免疫时分别用四君子汤和加味四君子汤煎剂按2.5，5.0，7.5 mL/L 3个添加量兑水自由饮用，连用4 d。于14，28和42日龄分别在鸡翅静脉采血，进行白细胞、淋巴细胞计数,α-醋酸萘酯酶染色(ANAE)和β-微量血凝抑制试验检测各项免疫指标，研究四君子汤及加味四君子汤对商品肉鸡免疫功能的影响。新城疫疫苗免疫时以2.5 mL/L剂量添加四君子汤或加味四君子汤，在14，28和42日龄均可极显著提高鸡的淋巴细胞数和血液中新城疫的抗体水平(P0.01)；四君子汤以5.0 mL/L剂量或加味四君子汤以2.5 mL/L剂量添加均能显著提高白细胞数和T淋巴细胞百分率 (P0.05)。四君子汤和加味四君子汤均能有效提高鸡的特异性免疫和非特异性免疫水平。

English. The study is to provide the basis for impoldering high quality and efficacy immunopotentiator of traditional Chinese medicine. 420 one-day old AA broilers were randomly divided into seven groups, 3 replicates for 20 broilers with in each.Broilers drank the water added supplementary sijunzi decoction or sijunzi decoction with the concentration of 2.5, 5.0 and 7.5 mL/L respectively for 4 days, and at the same time, they were immunized with Lasota. Then the numbers of white blood cell and lymphocyte were counted, HI antibody level and T lymphocyte percentage were tested in blood with ND-HI and ANAE on 14th, 28th and 42th d respectively to investigate the effect of sijunzi decoction and supplementary sijunzi decoction on immune function in broilers. When the broilers were immunized with Lasota, the numbers of lymphocyte and HI antibody level were improved (P0.01) significantly on 14th, 28th and 42thd in 2.5 mL/L sijunzi or supplementary sijunzi decoction group; Meanwhile, the numbers of white blood cell and T lymphocyte percentage were promoted (P0.05) significantly in 2.5 mL/L supplementary sijunzi decoction group and 5.0 mL/L sijunzi decoction group. Sijunzi decoction and supplementary sijunzi decoction can improve the level of specific and non-specific immunity effectively in broiler chickens.