The study on induction and proliferation of tube bulbs in Lilium brownii
2008
Liu Hongmei | Linghu Keyong | Fang Xiaobo
With different parts of bulb scale as explants, the proliferation method of Guizhou Lilium brownii were studies with 3% sodium hypochlorite and MS medium with different concentrations of hormones. The results showed that it is feasible to disinfect the bulbs of Lilium brownii with 3% sodium hypochlorite, moreover, the sodium hypochlorite is very cheap and harmless to researchers, experimental materials and environment. MS+NAA 0.3 mg/L + 6-BA 1.5 mg/L is optimum for the induction of bulbs, and the basal part of Lilium brownie is the optimum explants. After culture for 25 d on the same medium, the tube bulbs could obtained with the characteristics of high propagation coefficient, strong and new roots. The survival rate was over 90% for transplantation of tube bulbs with diameter between 1-2 cm. The method developed in the present study can proliferate abundant Lilium brownii seedling in short time.
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