Determination of emodin and chrysophanol contents in callus of Cassia tora L. leaf
2008
Zhou Jiayu | Liao Hai | Li Liping | Liu Le | He Kuibang | Ma Li
[Objective] Through inductive culture, emodin and chrysophanol contents in cassia seed were increased.[Method]MS culture medium was used to bourgeon seedlings of cassia seed and induce cotyledon callus of cassia seed while HPLC method was adopted to determine emodin and chrysophanol contents.[Result] Emodin and chrysophanol contents in cotyledon callus were 0.099% and 0.312% respectively while they were 0.029% and 0.190% respectively in cassia seed.[Conclusion]The method of inducing cotyledon callus were helpful for increasing emodin content in cassia seed.
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