Study on detecting methods on residual amount of melamine in chicken meat | 鸡肉中三聚氰胺残留量检测方法研究

Chinese. ［目的］为检测动物性食品中的三聚氰胺提供参考方法。［方法］以鸡肉匀浆为材料，用高效液相色谱法和气相质谱法检测其中的三聚氰胺含量，并对检测方法的回收率和精密度进行测定。［结果］高效液相色谱法确定的鸡肉中三聚氰胺的最低检测限为1 μg/g，最低定量限为2 μg/g，在0.05~50 μg/g范围内线性关系良好；方法的回收率大于80%，测定结果的日内变异系数小于7%，日间变异系数小于10%。气相质谱法确定的三聚氰胺的最低检测限为30 ng/g，最低定量限为5 030 ng/g，在5~500 ng/g范围内线性关系良好；方法的回收率在80%以上，测定结果的日内变异系数小于8%，日间变异系数小于10%。［结论］该研究为鸡肉中三聚氰胺的检测提供了新方法。_

English. ［Objective］The study was to provide a reference method for detecting melamine in animal food. ［Method］ With the homogenate of chicken meat as the material, the melamine amount in it was detected with high performance liquid chromatography (HPLC) and gas phase mass spectrometry method, and then the recovery rate and the precision of the 2 detection methods were determined. ［Result］ The minimum detection limit on melamine in chicken meat by high performance liquid chromatography (HPLC) was 1 μg/g, and the limit quantification was 2 μg/g, and in 0.05-50 μg/g, the linear relationship was good. The recovery rate of this method was over 80%, the within-day variation coefficient was less than 7%, and the inter-day variation coefficient was less than 10%. The minimum detection limit on melamine in chicken meat by gas phase mass spectrometry method was 30 ng/g, and the limit quantification was 5 030 ng/g, and in 5-500 ng/g, the linear relationship was good. The recovery rate of this method was over 80%, the within-day variation coefficient was less than 8%, and the inter-day variation coefficient was less than 10%.［Conclusion］The study provided a new method to detect melamine in chicken meat._