Effects of polysaccharides from Dendribum nobile on TNF-α and NO secretion in peritoneal macrophages of mice induced by LPS_ | 金钗石斛多糖对脂多糖诱导的小鼠腹腔巨噬细胞分泌TNF-α•NO的影响

Chinese. ［目的］研究金钗石斛多糖对脂多糖(LPS)诱导的小鼠腹腔巨噬细胞分泌肿瘤坏死因子α（TNF-α）、一氧化氮(NO)的影响。［方法］用不同浓度金钗石斛多糖作用于小鼠腹腔巨噬细胞， LPS作为诱导剂，采用放射免疫法检测细胞培养液中TNF-α的含量，硝酸酶还原法测定细胞培养液中NO的含量，荧光法测定细胞内诱导型一氧化氮合酶（iNOS）的活性，实时PCR（real-time PCR）法测定TNF-αmRNA、iNOS mRNA的表达。［结果］与LPS组比较，金钗石斛多糖在50~400 mg/L范围内可干预LPS对小鼠巨噬细胞的作用，使小鼠腹腔巨噬细胞TNF-α、NO合成减少，iNOS活性降低，TNF-αmRNA、iNOS mRNA的表达降低。 ［结论］ 金钗石斛多糖可改善LPS对小鼠腹腔巨噬细胞的作用，使TNF-αmRNA、iNOS-mRNA的表达降低，TNF-α、NO合成减少，从而起到抗炎的作用。_

English. ［Objective］ Study on the effects of Dendribum nobile polysaccharide on tumor necrosis factor-α (TNF-α) and nitric oxide (NO) release by mouse peritoneal macrophage induced Lipopolysaccharide (LPS) in vitro._［Method］ The peritoneal macrophages of mice were conducted with LPS in the presence of Dendribum nobile polysaccharide at various concentration .TNF-α was detected by radioimmunoassay, NO content of cell culture fluid was determined by nitrate reductase method, the activity of inducible nitric oxide synthase (iNOS) was measured by fluorimetry assay .The expression of TNF-α and iNOS mRNA were observed by real-time polymerase chain reaction (real-time PCR).［Result］ Compared with the control group, Polysaccharides of Dendribum nobile could markedly inhibit the secretion of TNF-α and NO and decrease the activity of iNOS and down-regulate the expression of TNF-α m RNA and iNOS mRNA in a dose dependent manner.［Conclusion］Dendribum nobile polysaccharide can improve TNF-α mRNA、iNOS mRNA and its protein expression induced by Lipopolysaccharide in mouse peritoneal macrophage that may play a very important role in the prevention of inflammation ._