Study on the conditions for producing cellulase by liquid fermentation with aspergillus niger HQ-1 | 黑曲霉HQ-1液体发酵产纤维素酶条件研究

Chinese. ［目的］ 探讨黑曲霉HQ-1液体发酵产纤维素酶的最适条件。［方法］以1株产酶活力相对较高的黑曲霉HQ-1为出发菌株，采用单因子试验和正交试验对黑曲霉HQ-1液体发酵产纤维素酶的最适条件进行初步研究，并测定了CMC酶（CMCase）和滤纸酶（FPAase）的活力。［结果］ 影响黑曲霉HQ-1液体发酵产CMCase和FPAase的因素依次是复合碳源的含量、2种碳源的比例、氮源含量和装液量。该菌产CMCase的最适培养基为：麸皮+玉米秸秆粉15‰（1∶1），NH4Cl 2.0‰，其他成分同Mandel’s营养液；产FPAase的最适培养基为：麸皮+玉米秸秆粉20‰（1∶1），NH4Cl 1.5‰，其他成分同Mandel’s营养液。2种培养基的最适pH值和装液量均分别为5.0和50 ml/250 ml三角瓶。在30 ℃和170 r/min下培养4 d后，CMCase和FPAase达到525.2和217.6 IU。［结论］ 该研究为纤维素酶生产菌株的研发提供一定的技术参考。

English. ［Objective］ The study aimed to discuss the optimum condition for producing cellulase by liquid fermentation of Aspergillus niger HQ-1. ［Method］ With 1 strain of A. niger HQ-1 with higher activity of producing cellulase as the original srtain, the optimum conditions for liquid fermentation of A. niger HQ-1 to produce cellulase was studied primarily through the single factor experiments and orthogonal tests and the activities of CMCase and FPAase were measured. ［Result］ The factors influencing the production of CMCase and FPAase by liquid fermentation of A, niger HQ-1 were in order were the content of compound carbon origin, the ratio of 2 kinds of carbon, the content of nitrogen and the liquid volume in flask. The optimal medium of producing CMCase was as follows: 15‰ bran+corn stalk powder (1∶1), 2.0‰ NH4Cl, other components as same as Mandel’s medium. The optimal medium of producing FPAase was as follows: 20‰ bran+corn stalk powder (1∶1), 1.5‰ NH4Cl, others were as same as Mandel’s medium. For both medium, the optimum pH was 5.0 and medium volume was 50 _ml/250_ ml triangle flask. The activities of CMCase and FPAase reached 525.2 and 217.6 IU respectively after culturing for 4 d under 30 ℃ and 170 r/min. ［Conclusion］This study provided some technique reference for the research and development of producing cellulase strain._