Isolation and identification of antifungal metabolites of streptomyces strain 4301 | 链霉菌4301菌株抑菌活性物质的分离与结构鉴定
2009
Chen Min, South China Agricultural University, Guangzhou(China) | Lan Jinfu, South China Agricultural University, Guangzhou(China), College of Sciences | Feng Minyan, South China Agricultural University, Guangzhou(China), College of Sciences
Chinese. 从土样中分离筛选的链霉菌4301菌株的代谢产物具有抑菌活性，通过溶媒萃取并利用各种柱层析手段从该菌株发酵液中分离得到5个化合物，利用波谱技术确定其结构分别为槲皮素、芹菜素、阿魏酸、香草酸和没食子酸，并初步评价了化合物I―V的抑菌活性．生测结果表明：浓度为10mmol/L槲皮素对香蕉炭疽病菌Colletotrichummusae、荔枝炭疽病菌Glomerella cingulata、荔枝霜疫霉菌Peronophythora litchii和小麦赤霉病菌Fusariumgraminearum均有抑菌作用．浓度为10mmol／L的香草酸对荔枝霜疫霉菌的抑菌圈直径达到了28mm．[著者文摘]
Show more [+] Less [-]English. Streptomyces sp. strain 4301 isolated from the soil displayed fungicidal activity. Five compounds were isolated and purified from fermented broth of the strain through silica gel column chromatography. Their structures were identified on the basis on their IR, MS,. H and . 3C NMR. The five compounds were quercetin,apigenin,ferulic acid and vanillic acid, gallic acid. The fungicidal activity of these compounds were assayed. Quercetin inhibited mycelial growth of four test plant pathogens including Colletotrichum musac, Glomerella cingulata ,Peronophythora litchii and Fusarium graminearum. The diameter of inhibitory cycles of P. litchii affected by vanillic acid at a concentration of 10 mmol/L was 28 ram.
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