Primary studies on the penetration and translocation of celangulin V in the plants | 苦皮藤素V在植物体内穿透与输导的初步研究

Chinese. 了解植物源杀虫活性物质苦皮藤素V在植物体内的穿透与输导作用。以蚕豆苗、小麦苗为供试植物材料，用苦皮藤素V处理植物根系及叶片，定期取样，采用高效液相色谱（HPLC）检测植物中的苦皮藤素V含量。苦皮藤素V可以穿透蚕豆苗根系进入植物体内，处理12 h后蚕豆苗根系中苦皮藤素V含量为0.20 μg/g，48 h后达到1.72 μg/g；苦皮藤素也可以穿透叶片进入植物体内，处理12 h后，蚕豆上层叶片苦皮藤素含量为0.34 μg/g，48 h后达到0.77 μg/g。水体中的苦皮藤素V可以穿透小麦苗根系进入植物体内，处理8 h后，植物中苦皮藤素V含量为0.29 μg/g，36 h后达到2.94 μg/g，但由于土壤对苦皮藤素V的吸附作用，小麦根系难以吸收到施于土壤的苦皮藤素V。苦皮藤素V可以穿透植物根系或叶片在植物体内输导。

English. The objective of this research was to comprehend the penetration and translocation of botanical insecticide celangulin V in the plants. The samples of broadbean or wheat seedling that treated by root system or leaf spraying were collected at regular intervals, and the content of celangulin V in samples was analyzed by high performance liquid chromatography. The results showed that celangulin V could be penetrated and translocated in the broadbean seedlings from the roots or leaves. The content of celangulin V in the samples was 0.20 μg/g 12 h after treatment, 1.72 μg/g 48 h after treatment by root absorption, and 0.34 μg/g 12 h after treatment, 0.77 μg/g 48 h after treatment by leaf absorption. The results also showed that celangulin V in water could be penetrated and translocated in the wheat seedlings from the roots. The content of celangulin V in the samples was 0.29 μg/g 8 h after treatment, 2.94 μg/g 36 h after treatment. No celangulin V was detected in the wheat seedling which was grown in soil treated with celangulin V. Celangulin V could be penetrated and translocated in the plant from roots or leaves.