Effects of the modified well of well culture system on the in vitro production of bovine embryos | 改进V形孔培养法对牛胚胎体外生产效果的影响

Chinese. 探索V形孔培养法(WOW)、改进V形孔培养法(mWOW)和常规微滴培养法对牛胚胎体外生产效果的影响。将牛卵母细胞和受精卵分别采用常规微滴培养法（对照组）、WOW和mWOW进行体外培养，对照组每个微滴中置入20～25枚卵母细胞或胚胎，WOW和mWOW组每个V形孔中置入1枚卵母细胞或胚胎，计算卵裂率、囊胚率及受精后第6，7，8天的囊胚总细胞数和囊胚孵化率。mWOW组的卵裂率、囊胚率及第6，7，8天囊胚的总细胞数、囊胚孵化率与常规微滴组差异不显著（P＞0.05），但显著高于WOW组（P＜0.05）；3个组第6，7，8天获得的囊胚总细胞数均呈逐渐下降的趋势。以TCM-199为基础培养液，用mWOW可以完成牛胚胎体外生产的全过程，且比WOW培养胚胎具有更强的发育能力和更好的质量。体外培养时，发育越快的胚胎质量越高。

English. The research was to study the effects of the well of well system(WOW), the modified well of well system(mWOW) and drop system on the development potential and quality of in vitro produced bovine embryos. The WOW, mWOW and drop were prepared before experiments. Bovine oocytes and presumptive zygotes were cultured in drop (control), WOW and mWOW systems respectively. About 20-25 oocytes or embryos were cultured in every one drop for control group. Only one oocyte or embryo was cultured in every V-shape well for WOW and mWOW groups. Cleavage rates, blastocyst rates and total cell number of embryos at 6, 7, and 8 days were examined. There was no significant difference for cleavage rates, blastocyst rates and total cell number of embryos at 6, 7, and 8 days between mWOW and drop group (P0.05). However, above indexes of both WOW and mWOW groups were higher (P0.05) than that of WOW group. In addition, the total cell number at 6, 7, and 8 days for the three tratments all declined gradually. In conclusion, the whole process of bovine embryo in vitro production could be finished in the mWOW system, and had better development potential and quality than the WOW system in TCM-199 medium. The faster the embryos developed in vitro cuture, the better the quality was.