The identifition of SRC- 1 gene by differential display RT-PCR related to intramuscular fat deposition in Sutai pigs | 苏太猪肌内脂肪沉积相关基因SRC-1的差异显示反转录PCR鉴定
2009
Liu Yanjuan, Nanjing Agricultural University, Nanjing(China), College of Veterinary Medicine | Tan Xun, Nanjing Agricultural University, Nanjing(China), College of Veterinary Medicine | Liu Jiaguo, Nanjing Agricultural University, Nanjing(China), College of Veterinary Medicine
Chinese. 采取90头18013龄去势苏太猪的背最长肌组织测定肌内脂肪含量，从中选取肌内脂肪含量差异极显著（P〈0．01）的最高和最低各6头组成RNA池，采用差异显示反转录PCR（DDRT―PCR）鉴定两组间差异表达的基因，结果共获得14条表达序列标签（expressedsequencetags），即EST序列，通过GenBank比对发现其中3条为已知序列。为进一步验证这3个已知基因与肌内脂肪之间的关系，从90个个体中选取肌内脂肪最高和最低各15个个体进行实时定量PCR检测。结果表明，细胞核受体共激活因子1基因（SRC-1）mRNA表达水平与肌内脂肪含量存在负相关关系（r=-0．38，P〈0．05）。提示：SRC-1蛋白有可能参与了肌内脂肪沉积的调节。[著者文摘]
Show more [+] Less [-]English. Longissimus dorsi （LD） muscle samples were collected from 90 castrated Sutai pigs aged 180 d and quantified intramus- cular fat （IMF） content. Two groups, each consisting of 6 individuals, of highest and lowest IMF content were selected to identi- fy the differential expressed genes between groups. Fourteen expressed sequence tags （EST） were gained and sequenced. The se- quences were compared with GenBank database. Three out of fourteen EST sequences were known genes. To further confirm the re- lation of these 3 genes with IMF content, 15 individuals with highest IMF content and 15 with lowest were exploded for mRNA ex- pression analysis by quantities real-time PCR. The mRNA levels showed significant inter-group difference for both storied receptor co-activator 1 gene （ SRC-1 ） （ P〈O. 05 ）. The result suggested that SRC-1 may be implicated in IMF deposition in pig LD muscle.
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