Effect of two kinds of insect protein source as substitute for fish meal on growth,body composition and protease activity of juvenile yellow catfish Pseud obugrus fulvidraco | 2种虫源性蛋白替代鱼粉对黄颡鱼幼鱼生长、体成分和蛋白酶活性的影响

Chinese. 以鱼粉作为对照,用黄粉虫、蚕蛹干粉半量和全量替代鱼粉,配制5组等蛋白质水平（40%）的饲料,对黄颡鱼幼鱼进行36 d的饲养.结果表明：对照组幼鱼的增重率、蛋白质消化率和饵料系数显著高于其他组（P〈0.05）,黄粉虫和蚕蛹全量替代组的增重率显著低于半量替代组（P〈0.05）;全黄粉虫组幼鱼的蛋白质消化率与2种半量替代组间的差异不显著（P〉0.05）,而蚕蛹组的蛋白质消化率显著低于其他替代组;除半量黄粉虫组幼鱼的饵料系数与对照组间的差异不显著外,全量替代组和半量蚕蛹组的饵料系数均显著高于对照组（P〈0.05）;除机体含水率外,各替代组幼鱼机体的粗蛋白含量只有全蚕蛹组显著低于其他组（P〈0.05）,而全蚕蛹组和半量蚕蛹组幼鱼的粗脂肪含量显著高于其他组（P〈0.05）;除半量黄粉虫组幼鱼的粗灰分含量与对照组间的差异不显著（P〉0.05）外,其他3组间的差异显著（P〈0.05）;对照组幼鱼肝胰脏、胃、肠道的蛋白酶活性均最高,除胃蛋白酶活性与半量黄粉虫组间的差异显著外（P〈0.05）,肝胰脏和肠道的蛋白酶活性与半量黄粉虫组间的差异均不显著（P〉0.05）,但显著高于2种全量替代组（P〈0.05）.[著者文摘]

English. Using yellow mealworm meal（YMM） and pupa meal（PM） as dietary protein to replace fish meal at two levels of 50%（50YMM 50PM） and 100%（100YMM 100PM）,with 100% fish meal as a control group,five kinds of feeds were prepared,which were the same protein level 40%.A 36 day feeding trial was carried out to examine growth parameters and feed utilization,body composition and protease activity in the juvenile yellow catfish（initial body weight 1.52±0.29 g・fish-1） fed with those formulated feeds.The results showed that there were significant differences in protein digestibility and feed efficiency between the control group and the other groups of juvenile yellow catfish（P0.05）.The weight gain ratio was significantly lower in the fish fed with the 100YMM and 100PM than the fish fed with 50YMM and 50PM.There was no significant difference in protein digestibility among the fish fed with the formulated feeds of 100YMM,50YMM and 50PM（P0.05）; The fish fed with 100PM had significantly lower protein digestibility than the other groups.Excepting no significant differences in feed efficiency between fish fed with 50YMM and control group（P0.05）,there were significantly higer feed efficiency in the fish fed with 100YMM,100PM and 50PM than the fish in control group（P0.05）.Except the body water content,only the crude protein of fish with fed 100PM was signifcantly lower than the other groups（P0.05）,but there was no significant difference among the fish with fed 50YMM and 100YMM（P0.05）.The crude fat of fish fed with 100PM and 50PM was signifcantly higer than the other groups（P0.05）.Excepting no significant difference in the crude ash between 50YMM and control group（P0.05）,there were significant difference among the other groups（P0.05）.The protease activities in hepatopancreas,stomach and intestine of yellow catfish juvenile in control group were highest among fish fed with the formulated feeds.Only pepsin activity was significantly different,but there was no significant difference between 50YMM and control group（P0.05）.And the protease activity was significantly higher in hepatopancreas and intestine of fish in control group than in 50YMM and 50PM（P0.05）.[著者文摘]