6 kinds of isozymes after long-term subculture of Emmenopterys henryi Oliv. | 濒危植物香果树愈伤组织经长期继代后的6种同工酶研究
2010
Du Jingchuan, Beijing Forestry University, Beijing (China), College of Biological Sciences and Biotechnology | Jing Danlong, China Three Gorges University, Yichang (China), Biotechnology Research Center | Chen Faju, China Three Gorges University, Yichang (China), Biotechnology Research Center
[Objective] The aim was to study the changes of zymography in 6 kinds of isozymes after long-term subculture of Emmenopterys henryi Oliv.. [Method] Non-denaturing polyacrylamide gel electrophoresis was used to analyze isozyme patterns such as esterase (EST), acid phosphatase (ACP), ATP enzyme (ATPase), amylase (AMY), superoxide dismutase (SOD) and peroxidase (POD) in long-term subculture callus of Emmenopterys henryi Oliv. [Result] The research showed that there were differences among the 6 kinds of isozymes in embryogenic callus and non-embryogenic callus of Emmenopterys henryi Oliv., and both levels could be taken as the basis for identification, the EST, ACP, and POD of non-embryogenic callus were significantly higher than embryogenic callus. The browning of non-embryogenic callus was non-level in the AMY, SOD and POD isozymes when was compared with normal non-embryogenic callus, while the EST, ACP and ATPase isozymes decreased; When the browning of embryogenic callus was contrasted with normal embryogenic callus, EST isozyme increased and the other 5 kinds of enzymes decreased. [Conclusion] The study provided theoretical basis for research morphological difference and browning of long-term tissue culture of Emmenopterys henryi Oliv..
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