Optimization of cell line building for Taxus chinensis var. mairei | 南方红豆杉细胞体系建立的优化
2010
Yuan Mengru, Nanjing Agricultural University, Nanjing（China）, Life Sciences College | Shen Bin, Nanjing Agricultural University, Nanjing（China）, Life Sciences College | Sun Yuxia, Nanjing Agricultural University, Nanjing（China）, Life Sciences College
[Objective] The paper aims to optimize the abduction and domestication conditions of Taxus chinensis var. Mairei callus. [Method] We compared the efficiencies of callus between different explants and investigated the multiplication conditions of callus and suspended cell culture conditions with the buds, young stems and young leaves from T. chinensis var as the explants. [Results] The effect was the best with the bud as the explants; the best way for sterilizing the explants of T. chinensis var mairei was: streptomycin detergent for 2 h + suds for 3 h + 75% alcohol disinfection for 30 s + 10% sodium hypochlorite solution for 25 min + 1‰ mercury chloride for 10 min; the optimum formula of callus subculture was: B5 + 4.0 mg/L NAA + 0.5 mg/L 2,4-D + 0.2 mg/L GA + 0.5 mg/L 6-BA + 2g/L AC. [Conclusion] This research built the high efficient regeneration system of T. chinensis var.
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