Study on the anti-MRSA strain B2′s physics and chemistry and its antagonistic activity | 抗MRSA活性菌株B2的理化特性和抑菌效果研究
2010
Zhang Xiaoyan, University of Electronic Science and Technology of China, Dalian (China), Zhongshan Institute | Liu Yuhuan, University of Electronic Science and Technology of China, Dalian (China), Zhongshan Institute
Chinese. ［目的］确定既适合B2菌株生长又能产生较强活性物质的培养条件。［方法］在不同的温度、pH值、NaCl浓度和各种碳源培养条件下，进行菌体生长状况研究和抑菌活性检测。［结果］ 菌株B2在4~37 ℃、pH值2.0~11.0、NaCl浓度为1%~6%，以葡萄糖、麦芽糖、乳糖、蔗糖、淀粉为碳源时都能生长，其最适生长温度为28 ℃，最适pH值为7.0，最适NaCl浓度为1%，最适生长碳源为麦芽糖。［结论］当pH值为5.0、温度为28 ℃，以麦芽糖作唯一碳源时，产生的拮抗物质活性最强。
Show more [+] Less [-]English. ［Objective］The purpose was to find the suitable culture conditions for strain B2 and producing antagonistic substance.［Method］ The anti-MRSA strain B2’s culture conditions such as different temperature、pH value and concentration of NaCl Cultivation of variais arbon sources were studied and its antagonistic activity was analysed.［Result］These tests showed that the suitable temperature for bacterium growth and producing anti-MRSA substance was 28 ℃, the suitable pH value for bacterium growth was 7.0, but 5.0 for producing anti_MRSA substance, the suitable concentration of NaCl for growth was 1%, the suitable carbon source for B2 growth and producing antagonistic substance was maltose.［Conclusion］The suitable culture conditions for strain B2 and antagonistic activity is that the temperature was 28 ℃,pH value 5.0,the carbon sourcefor was maltose._
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