Analysis of transgenic wheat by nested PCR_ | 巢式PCR检测转基因小麦的研究_
2010
Zhuang Hongtao, Hunan Agricultural University, Changsha (China), College of Bioscience and Biotechnology | Zhang Xuewen, Hunan Agricultural University, Changsha (China), College of Bioscience and Biotechnology | Zhang Zengyan, Chinese Academy of Agricultural Sciences, Beijing (China), Crop Science Institute
Chinese. 利用巢式PCR方法对转RSSP1-TiERF1基因的T0代小麦进行了DNA检测。结果表明，该方法对于检测基因组庞大、外源基因序列GC含量高且与内源基因同源性高的转基因小麦十分有效。
Show more [+] Less [-]English. Nested PCR method was used in DNA analysis of transgenic wheat. The results indicated that this method was very useful when the transgenic plant had large genome, the foreign gene was with high content of GC and high homology to the genome. _
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