Research of anthocyanin composition in blue and purple wheat grains | 蓝、紫粒小麦籽粒花色苷组成分析

Chinese. 【目的】应用超高效液相色谱配以串联质谱技术和二极管阵列检测技术对蓝、紫粒小麦籽粒中的花色苷进行分离与鉴定,揭示蓝、紫粒小麦籽粒花色苷组成成分。【方法】小麦籽粒花色苷用90%甲醇水溶液(含0.5%甲酸)超声波提取,SPE C18柱净化处理。应用超高效液相色谱串联质谱对蓝、紫粒小麦籽粒中的花色苷提取物进行母离子扫描,初步确定蓝、紫粒小麦籽粒中的花色苷种类,用全扫描、子离子扫描,多反应检测技术对蓝、紫粒小麦籽粒中的花色苷组分和含量进行分析。【结果】蓝、紫粒小麦籽粒中含有14种不同种类的花色苷类化合物,且不同的蓝、紫粒小麦籽粒中花色苷的种类与含量不同。【结论】明确了蓝、紫粒小麦籽粒中花色苷的组分与含量,建立了应用质谱快速分离与鉴定蓝、紫粒小麦籽粒中花色苷的方法。

English. 【Objective】 A systematic method for anthocyanin identification using tandem mass spectrometry (MS/MS) coupled to Ultra performance liquid chromatography (UPLC) with photo-diode array detection (PDA) was developed. Isolation and identification of anthocyanins from blue, purple wheat grains were made, and then the compositions of anthocyanin were revealed. 【Method】 Wheat grains were extracted by 90% methanol-water solution (containing 0.5% formic acid), ultrasonic assisted extraction was employed, and purified on SPE C18 column. The precursor ions of anthocyanins were scanned using UPLC/MS/MS on a triple quadrupole instrument allowing for the specific determination of each category of anthocyanins. Further characterization of each anthocyanin was performed using MS/MS product-ion scanning, full scanning and MRM (multiple reaction monitoring). 【Result】 Fourteen anthocyanins were identified in blue and purple wheat grains. Significant difference was found in the composition and content of anthocyanins among different wheat varieties. 【Conclusion】 The study has defined the compositions and contents of anthocyanins in blue and purple wheat grains. The present approach allows mass spectrometry to be used as a highly selective detector for rapid identification and characterization of anthocyanins in wheat grain.