Generation and identification of 5No. chinese cabbage-cabbage disomic alien addition lines | 大白菜-结球甘蓝5号二体异附加系的选育及鉴定

Chinese. 【目的】获得稳定遗传的大白菜-结球甘蓝二体异附加系。【方法】以大白菜-结球甘蓝5号单体异附加系为材料,对其自交后代进行细胞学及SSR鉴定。【结果】37株自交后代中2n=22植株的比率为10.81%,通过对4个2n=22的植株进行核型分析及SSR鉴定,确定其为大白菜-结球甘蓝5号二体异附加系。减数分裂观察发现,大白菜-结球甘蓝5号二体异附加系后期Ⅰ虽然出现染色体落后现象,但在后期Ⅱ中染色体的分裂方式以11-11- 11-11为主,其比率为69.55%。【结论】获得了大白菜-结球甘蓝5号二体异附加系,其外源染色体与甘蓝07连 锁群相对应;该二体异附加系减数分裂形成的四分体以附加1条的染色体为主(n=11),有较高的遗传稳定性。二体异附加系的获得为研究芸薹属A、C基因组的亲缘关系,以及向大白菜中导入结球甘蓝的优良基因具有重要 意义。

English. 【Objective】 The objective of this study is to obtain homozygous Chinese cabbage-cabbage disomic alien addition lines. 【Method】 In this study, 5No.Chinese cabbage-cabbage monosomic addition line was used, and its selfing F1 generation was tested by means of identification of cytology and SSR analysis. 【Result】 The results showed that the ratio of disomic addition lines (2n=22) was 10.81% in 37 screened plants of selfed progeny. Through karyotype analysis and molecular marker identification, 4 Chinese cabbage-cabbage disomic alien addition lines (Chinese cabbage with 5No. chromosome from cabbage) were identified in the plants with 2n=22. The alien chromosomes of disomic alien addition lines moving late was observed at anaphaseⅠof meiotic behavior, but the distribution of most of the chromosomes was 11-11-11-11 at anaphaseⅡ, the ratio was 69.55%. 【Conclusion】 5No. Chinese cabbage-cabbage disomic alien addition lines were obtained. The alien chromosome of 5No. Chinese cabbage-cabbage disomic alien addition lines corresponded with 07 linkage group of cabbage. Disomic alien addition lines undergo meiosis to form the tetrad which is mainly additional one chromosome of the cabbage (n=11), it has higher hereditary stability. The acquisition of alien addition lines for the study of the phylogenetic relationships between brassica A and C genome, and for transmitting valuable genes from cabbage to Chinese cabbage and expanding the genetic background of Chinese cabbage germplasm, are of great significance.