Micropropagation of cranberry (Vaccinium macrocarpon) through shoot tip cultures - Short communication
2011
Sedlak, J.,Vyzkumny a Slechtitelsky Ustav Ovocnarsky, Holovousy (Czech Republic) | Paprstein, F.,Vyzkumny a Slechtitelsky Ustav Ovocnarsky, Holovousy (Czech Republic)
The goal of this study was to determine an efficient micropropagation system for Vaccinium macrocarpon. Cranberry cultivar Howes was successfully established in vitro using mercuric chloride in a concentration of 0.15% as a sterilization solution. Anderson's rhododendron medium (AN), half-strength Murashige and Skoog medium and McCown woody plant medium (WPM) containing the cytokinin zeatin in concentrations 0.5, 1 or 2 mg/L were tested. Generally, the highest multiplication rate (2.7) was obtained for cv. Howes on AN medium with zeatin (1 mg/L). The effects of indole-3-butyric acid (IBA), indole-3-acetic acid (IAA) and naphtalen acetic acid (NAA) on root induction were tested in WPM medium. The percentage of rooting was 100% for NAA, 86% for IBA and 81% for IAA. From the tested variants, NAA also promoted the highest development of good quality roots (more than 5) per shoot without callus formation.
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