Rapid molecular identification and characteristics of Lactobacillus strains
2010
Markiewicz, L.H.,Polish Academy of Sciences, Olsztyn (Poland). Inst. of Animal Reproduction and Food Research | Biedrzycka, E.,Danisco Biolacta, Olsztyn (Poland) | Wasilewska, E.,Polish Academy of Sciences, Olsztyn (Poland). Inst. of Animal Reproduction and Food Research | Bielecka, M.,Polish Academy of Sciences, Olsztyn (Poland). Inst. of Animal Reproduction and Food Research
Eleven type strains and 24 Lactobacillus isolates, preliminarily classified to the species due to phenotypic features, were investigated. Standard methods of identification with species-specific PCRs and typing with PFGE (with ApaI, NotI and SmaI restriction enzymes) allowed us to distinguish 16 unique strains belonging to 5 species (L. acidophilus, L. delbrueckii ssp. bulgaricus, L. plantarum, L. rhamnosus, L. salivarius). Alternative approach with 16S-23S rDNA ARDRA identification (with merely two restrictases, BsuRI and TaqI) and PCR-based typing (RAPD with two random- and rep-PCR with (GTG)(5) primers) showed to be more discriminative, i.e. 21 unique strains were classified in the same species as above. As a result, 7 out of 24 phenotypically species-assigned isolates were reclassified. The alternative procedure of rapid identification and typing of Lactobacillus isolates appeared to be equally effective and shortened from 1 week to 2-3 d (in comparison to the standard methods).
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