Antioxidant activity of enzymatic system of two different wheat (Triticum aestivum L.) cultivars growing under salt stress
2011
Bhutta, W.M.,University of Agriculture, Faisalabad (Pakistan). Centre of Advanced Studies in Applied Genetics and Saline Agriculture
Two wheat genotypes, S-24 - salt tolerant and DN-27 - salt sensitive, were used in order to study the effect of different salinity levels on some physiological and agronomic wheat characters. Salt was added to the nutrient solution starting on the 15th day after germination by 25 mol/cubic m NaCl/day to a final level of 80 and 160 mol/cubic m NaCl. Relative water content, membrane stability index and the activities of superoxide dismutase, peroxidase and catalase were determined after 20 and 40 days of salt stress exposure. Enzyme activities increased in S-24 with the increase of salt stress, while in DN-27, all the enzymes showed constant activities at all stress levels. The values of relative water content and membrane stability index decreased with increasing salt concentrations. It can be concluded that all three antioxidant enzymes were limiting, which led to the salt sensitivity in wheat genotype DN-27.
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