Distinguishing grapefruit and pummelo accessions using ISSR markers
2010
Uzun, A.,Erciyes Univ., Kayseri (Turkey). Dept. of Horticulture | Gulsen, O.,Erciyes Univ., Kayseri (Turkey). Dept. of Horticulture | Yesiloglu, T.,Cukurova Univ., Adana (Turkey) Dept. of Horticulture | Aka-Kacar, Y.,Cukurova Univ., Adana (Turkey) Dept. of Horticulture | Tuzcu, O.,Cukurova Univ., Adana (Turkey) Dept. of Horticulture
Inter-Simple Sequence Repeat markers were used to distinguish twenty-nine grapefruit (Citrus paradisi), five pummelo (Citrus maxima) and one Citrus hassaku accessions. Twelve ISSR primers produced a total of 100 fragments and 62 of them were polymorphic. The number of average polymorphic fragments per primer was 5.2. The mean polymorphism information content was 0.37. The unweighted pair group method arithmetic average analysis demonstrated that the accessions had a similarity range from 0.79 to 1.00. The accessions were separated into two main clusters; group A with five pummelos and group B with grapefruits. In the pummelo cluster, all pummelos were distinguished, whereas in the grapefruit cluster some accessions were not clearly separated. There was a low level of variation in the grapefruits due to their mutation origin.
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