Combined drought and heat stress in wheat: changes in some heat shock proteins
2011
Grigorova, B.,Bulgarian Academy of Sciences, Sofia (Bulgaria). Inst. of Plant Physiology | Vaseva, I.,Bulgarian Academy of Sciences, Sofia (Bulgaria). Inst. of Plant Physiology | Demirevska, K.,Bulgarian Academy of Sciences, Sofia (Bulgaria). Inst. of Plant Physiology | Feller, U.,University of Bern (Switzerland). Inst. of Plant Sciences and Oeschger Centre for Climate Change Research
The influence of combined and individually applied drought and heat stress was studied in two wheat cultivars: resistant cv. Katya and susceptible cv. Sadovo. Relative water content decreased and electrolyte leakage increased due to individual and combined application of both stresses. Initial heat shock protein profile has been outlined via SDS electrophoresis of leaf extracts. The results obtained were confirmed by immunoblotting with anti-HSP70 monoclonal antibodies, anti-HSP110 polyclonal antibodies and anti-alphabeta-crystalline polyclonal antibodies. The effect of simultaneously applied water stress and heat shock resembled the alterations in protein expression provoked only by water stress and differed significantly from the changes occurring after the individual application of heat stress.
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