Shoot regeneration in tissue cultures of Globe artichoke, Cynara scolymus L.
2009
Khalil, H. M.M. | Khalil, M.A.I. | El-Shabasi, M.S.S. | Ismail, H.E.M.
This investigation was conducted to study the effect of different concentrations of kinetin (0.0, 0.5, 1.0, 2.0, 3.0 and 4.0mg/1) on the morphogenic respone of globe artichoke through tissue culture technique. All used kinetin concentrations caused a marked significant effect on both number of shoots and leaves per explant at 15,30 and 45 days of culture except number of leaves/ explant of 15 days of culture, as well as the fresh weight of shoots and average dry weight of shoot at 45 days of culture. Kinetin at 0.5mg/I followed by kinetin at 2.0 mg/l. recoded the highest values of both number of shoots and leaves per explant. On the other hand, kinetin at lmg/1 being the most effective and favourable treatment for increasing the number of leaves per shoot. Moreover, kinetin at 0.5 mg/l was found to be the optimum concentration regarding fresh weight of shoots. In addition, the maximum values of average dry weight of shoot were more achieved via application of kinetin to MS-medium at a concentration of 3.0 mg/L.
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