Phylogenetic analysis of some indigenous cyanobacterial strains by random amplified polymorphic dna-pcr
2011
Ali, A.S. | Salem, S.H. | El-Zamik, F.I. | Abd El-Basit, M.L.
Molecular characterization for 11 cyanobacterial strains belonging to 3 genera namely Nostoc (4 strains of N. muscorum and 3 strains of N. calcicola), Anabaena (one strain of A. oryzae and one strain of A. oscillarioides) and Calothrix (2 strains of C. clavata ) was done using the RAPD-PCR technique. Genomic DNA was extracted from these strains and amplified using the primers OPG 05, A- 01, OPV, OPT-07 and OPS-19 and distinct RAPD-PCR fingerprints were generated. Unique banding patterns were observed from all tested cyanobacterial strains and their molecular weights of each band were used to calculate the genetic distance among them, and the phylogenetic relations among these isolates was studied. RAPD fingerprinting results clearly showed the existence of wide range of genetic variation among the cyanobacterial strains. Data showed that phylogenetic relatedness between cyanobacterial strains was not highly influenced by with soil texture.
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