Effect of gamma irradiation in vitro bulbiets storage ability and genetic variation of six garlic genotypes
2010
El-Sayed, S.F. | Gharib, A.A. | El-Shamy, M.R. | Abd El-Wadod, N.A.
This investigation was carried out in the Tissue Culture Laboratory of Potato and Vegetatively Propagated Crops, Vegetable Research Department, Agricultural Research Center (A.R.C.), during the period of 2005-2008 to study the effect of different doses of gamma rays (0, 3, 5, 8, 10, 12 and 14 Gy) on the in vitro bulblets production from shoot basal plate (Sbp) explants of six garlic genotypes including three cultivars (Balady, American and Omani) and three clones (Sids-40, EgaSeedl and EgaSeed2) on a bulblets production medium (MS + 120 g/1 sucrose + 5 g/l activated charcoal + 0.1 mg BA/1). The present investigation also studied the storage ability of the in vitro produced bulblets stored in a growth chamber at 25° C during four months of storage. The random amplified polumorphic DNA (RAPD) analysis confirmed the genetic background of irradiated garlic genotypes. All garlic genotypes were able to produce bulblets from Sbp explants. At the end of storage period (4 months) the bulblets were healthy and suitable for cultivation in the field. RAPD analysis indicated that the six garlic genotypes are different in the number of bands and this was attributed to the gamma ray doses.
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