Soluble fibre quantification using different gravimetric methodologies | Cuantificación de la fibra soluble mediante distintas metodologías gravimétricas
2011
Abad, R., Universidad Politécnica de Madrid (España). Escuela Técnica Superior de Ingenieros Agrónomos | Carabaño, R., Universidad Politécnica de Madrid (España). Escuela Técnica Superior de Ingenieros Agrónomos | Delgado, R., Universidad Politécnica de Madrid (España). Escuela Técnica Superior de Ingenieros Agrónomos | Menoyo, D., Universidad Politécnica de Madrid (España). Escuela Técnica Superior de Ingenieros Agrónomos | García, J., Universidad Politécnica de Madrid (España). Escuela Técnica Superior de Ingenieros Agrónomos
The aim of this work was to evaluate different methods to quantify dietary soluble fibre in four diets and six feedstuffs with a wide range of soluble fibre content (from sugar beet pectin to lignocellulose). AOAC methods were used to determine total, insoluble and soluble dietary fibre (AOAC, 985.29 for total dietary fibre, TDF, and 991.43 for insoluble and soluble dietary fibre, IDF and SDF), and soluble fibre was also obtained by difference: TDFNDF (amylase treated and protein and ash corrected), TDF-IDF and TDF- rDMSiv sub(12) (residue of in vitro ileal dry matter digestibility). The major proportion of insoluble fibre was recorded by rDMSiv sub(12) whereas NDF the lowest (538 vs. 484 g/kg DM), showing IDF an intermediate value (P = 0.012). It implied that the highest value of soluble fibre calculated by difference (total fibre - insoluble fibre) was that obtained with TDF-NDF whereas lower and similar values were obtained using TDF-IDF and TDF- rDMSiv sub(12) (165 vs. 132 y 111 g/kg DM, respectively). However, none of the values obtained by difference differed with the direct method (SDF, 139 g/kg DM). Soluble fibre values for each feed depend on the methodology used. For sugar beet pectin soluble fibre values obtained by difference were similar but lower than the SDF value, whereas for sugar beet pulp a different value was obtained with each methodology. It is necessary to find what methodology is better correlated with the physiological effects on the animal to propose the best choice to quantify soluble fibre.
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