Level of transcription of genes involved in apoptosis and competence of prepubertal bovine oocytes in vitro matured with leptin | Efecto de la adición de leptina al medio de maduración in vitro sobre el nivel de transcripción de genes involucrados en la apoptosis y la competencia de ovocitos de terneras prepúberes
2011
Córdova, B., Universitat Autònoma de Barcelona, Bellaterra (España). Dept. de Medicina y Cirugía Animales | Morató, R., Universitat Autònoma de Barcelona, Bellaterra (España). Dept. de Medicina y Cirugía Animales | De Frutos, C., Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Madrid (España) | Bermejo Álvarez, P., Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Madrid (España) | Gutiérrez Adán, A., Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Madrid (España) | Mogas, T., Universitat Autònoma de Barcelona, Bellaterra (España). Dept. de Medicina y Cirugía Animales
Leptin has been shown to exert positive effects during in vitro maturation of adult bovine oocytes, influencing blastocyst development, apoptosis and the transcript levels of developmentally important genes. The present study was performed to analyze the differential effects of leptin on prepubertal calf cumulus oocyte complexes. In particular, we evaluated whether it affects the transcript levels of genes in oocytes and cumulus cells. Cumulus-oocyte complexes were matured in serum-free medium containing 1 mg/ml polyvinyl alcohol (PVA) and 10, 100 and 1000 ng/ml leptin or in medium supplemented with 10% foetal calf serum (FSB) as a positive control. Relative mRNA levels of seven genes were evaluated by qRT-PCR, and five (TP53, BAX, DNMT3A, PGTS2 and LEPR) showed differences among specific groups. LEPR mRNA abundance was significantly higher in the group matured with FCS compared with the other groups. Two genes involved in the apoptosis pathway showed significant differences between specific groups, suggesting an antiapoptotic effect of 10 ng/ml leptin in oocytes (TP53) and 100 ng/ml in cumulus cells (BAX). In conclusion, the addition of leptin to the in vitro maturation medium of prepubertal bovine oocytes exerts an antiapoptotic effect in the oocyte and cumulus cells and decreases the mRNA abundance of leptin receptor (LEPR).
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