Evaluation of methanolic extract of Allium sativum and Saussurea costus in yaks with infectious keratoconjunctivitis
2010
BANDYOPADHYAY, SAMIRAN | BISWAS, T K | SASMAL, D | SAMANTA, I | GHOSH, M K
The present study was undertaken to evaluate the efficacy of crude methanolic extract of fresh bulb of Allium sativum (G-MeOH), and roots of Saussurea costus (SC-MeOH) in yaks (Poephagus grunniens) with infectious keratoconjunctivitis. Bactericidal activity of G-MeOH and SC-MeOH was evaluated against Moraxella bovis and Neisseria spp isolated from eyes of yaks and yak hybrid with infectious keratoconjunctivitis and flies (Musca domestica). Following single disc diffusion assay, 5 resistant isolates of both bacteria were included for further study. Single disc diffusion and broth dilution method were used to determine the inhibitory effect of G-MeOH and SC-MeOH. Both the extracts displayed dose dependent antibacterial effect against all the isolates. Thereafter, the diluted plant derived products were subjected to clinical trials. Yaks (18) with infectious keratoconjunctivitis were included for clinical trial. Following application of the extracts, 8 yaks out of 12 (5 cured by G-MeOH, 3 cured by SC-MeOH) were completely cured within 7 days. However, 6 yaks where no extract was applied did not show any recovery. G-MeOH (83.33%: 5/6) exhibited the most prominent clinical efficacy followed by SC-MeOH (50%: 3/6). The results supported the traditional wisdom of herbal remedy use and suggested a potential clinical testing for G-MeOH and SC-MeOH on infectious keratoconjunctivitis.
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