Production of Mycelial Biomass and Exo-polymer by Hericium erinaceus CZ-2: Optimization of Nutrients Levels using Response Surface Methodology
2010
Cui, Fengjie, Jiangsu University, Zhenjiang, China | Liu, Zhiqiang, Zhejiang University of Technology, Hangzhou, China | Li, Yin, North Dakota State University, Fargo, USA | Ping, Lifeng, Zhejiang Academy of Agricultural Sciences, Hangzhou, China | Ping, Liying, Zhejiang University of Technology, Hangzhou, China | Zhang, Zhicai, Jiangsu University, Zhenjiang, China | Lin, Lin, Jiangsu University, Zhenjiang, China | Dong, Ying, Jiangsu University, Zhenjiang, China | Huang, Daming, Jiangsu University, Zhenjiang, China
The Doehlert experimental design was used to optimize the production of mycelial biomass and exo-polymer from Hericium erinaceus CZ-2 in this study. Statistical analysis showed that the linear and quadric terms of 3 variables: corn flour, yeast extract, and corn steep liquor had significant effects. The optimized combination of these 3 variables was confirmed through validation experiments. The optimal conditions for higher production of mycelial biomass (19.92 g/L) were estimated when the media composition concentrations were set as: 30.85 g/L, corn flour; 2.81 g/L, yeast extract; 16.9 mL/L, corn steep liquor; 10 g/L, glucose; 1 g/L, KH₂PO₄; and 0.5 g/L, MgSO₄ㆍ7H₂O; while a maximal exo-polymer yield (1.653 g/L) could be achieved when setting concentrations of: 32.71 g/L, corn flour; 2.35 g/L, Yeast extract; 14.42 mL/L, Corn steep liquor; 10 g/L, glucose; 1 g/L, KH₂PO₄; and 0.5 g/L, MgSO₄ㆍ7H₂O. The upscale production was also investigated using a 15 L fermentor using the optimized medium.
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