Microwave Irradiation-assisted RNA Extraction from Woody Tissues for Plant Virus Detection
2010
Duong, Thanh Van, Catholic University of Daegu, Gyungsan, Republic of Korea | Shin, D.I., Catholic University of Daegu, Gyungsan, Republic of Korea | Park, H.S., Catholic University of Daegu, Gyungsan, Republic of Korea
Plant tissue homogenization using a mortar or mechanical equipment has been the preferred method for obtaining high yields of total RNA; this method, however, is both time-consuming and expensive. Additionally, homogenization may generate excessive endogenous RNases, polyphenolics, and other substances that reduce the quality and quantity of RNA. In this study, we describe the microwave irradiation-assisted RNA extraction (MIRE) technique which, without tissue disruption and homogenization, allows for the cost-effective and rapid generation of intact RNA from apple cane shavings and the reliable detection of apple virus by RT-PCR.
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