Selection of Peptides Binding to HCV E2 and Inhibiting Viral Infectivity
2010
Hong, H.W., Hankuk University of Foreign Studies, Yongin, Republic of Korea | Lee, S.W., Dankook University, Yongin, Republic of Korea | Myung, H.J., Hankuk University of Foreign Studies, Yongin, Republic of Korea
The envelope glycoprotein E2 of hepatitis C virus (HCV) binds to various cell surface receptors for viral infection. We performed biopanning against this protein and selected peptides from phage display peptide libraries. Two short peptides, pep7-1 and pep12-1, were selected and their ability to inhibit the infection process was investigated. When pep7-1 was present, the infectivity of HCV particles in cell culture was notably decreased. This decrease was demonstrated by Western blot analysis, immunofluorescence assay, and reverse transcription PCR assay. However, pep12-1 showed little inhibitory effect on HCV infection.
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