Application of in-vitro mutation techniques for sugarcane improvement
2010
Khan, S.J. (Gomal Univ., Dera Ismail Khan (Pakistan). Faculty of Agriculture) | Khan, M.A. (Gomal Univ., Dera Ismail Khan (Pakistan). Faculty of Agriculture)
Three sugarcane mutants developed from the callus (CP43/33) subjected to four different radiation doses (0, 0.5, 2.0 and 4.0 krads) were tested in RCBD in the Faculty of Agriculture, Gomal University, Dera Ismail Khan, NWFP (now Khyber Pakhtunkhwa), Pakistan during 2005-06. Data were recorded on germination percentage, number of tillers per plant, stripped cane height, millable weight per cane, and stripped cane yield and sugar recovery. Highly significant differences were observed for all parameters. The lowest radiation dose 0.5 Krads proved to be the best to produce mutant plants with improved agronomic characteristics as compared to non-irradiated (somaclones) plants. The plants developed from non-irradiated callus proved inferior regarding all characters. The mutant plants developed from callus subjected to the highest radiation dose (4.0 krads) showed minimum performance for all parameters.
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