Optimization of the protocols for callus induction, regeneration and acclimatization of sugarcane cv. Thatta-10
2009
Ather, A. (Karachi Univ. (Pakistan). H.E.J. Research Inst. of Chemistry) | Khan, S. (Karachi Univ. (Pakistan). H.E.J. Research Inst. of Chemistry) | Rehman, A. (Karachi Univ. (Pakistan). H.E.J. Research Inst. of Chemistry) | Nazir, M.
A very efficient protocol for the micropropagation of indigenous sugarcane cultivar Thatta-10 is presented here. The axillary buds and shoot apices taken as an explant were surface sterilized with 20% Sodium hypochlorite solution for 20 minutes followed by initiation on simple MS medium. The initiated plantlets were then used for the callus induction experiment in which high amount of calli (100%) were produced using 2,4-D in the concentration of 3.0 mg/L with MS medium. The induced calli were further evaluated for the regeneration. Medium comprised of MS basal salts and vitamins with BAP (6-Benzyl Amino Purine) in the concentration of 1.0 mg/L, was found best in terms of highest number of shoot regeneration (31.50 plus minus 6.23). Maximum shoot elongation (10.52 plus minus 1.88) along with the highest number of root emergence (6.51 plus minus 2.41) was observed on the medium composed of MS salts and vitamins with GA (Gibberellic Acid) in the concentration of 3.0 mg/L. The In vitro grown plantlets were then transferred to green house conditions where they survived successfully with the rate of 96% (calculated after four weeks of transfer) when farm yard manure was used with garden soil in the ratio of 2:8 as a potting mixture.
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