Molecular evaluation of genetic variability of wheat elite breeding material | Molekularna evaluacija genetičke varijabilnosti elitnog oplemenjivačkog materijala pšenice
2009
Brbaklić, Lj., Institut za ratarstvo i povrtarstvo, Novi Sad (Serbia) | Kondić-Špika, A., Institut za ratarstvo i povrtarstvo, Novi Sad (Serbia) | Trkulja, D., Institut za ratarstvo i povrtarstvo, Novi Sad (Serbia) | Kobiljski, B., Institut za ratarstvo i povrtarstvo, Novi Sad (Serbia)
Estimation of genetic variability of breeding material is essential for yield improvement in wheat cultivars. Modern techniques based on molecular markers application are more efficient and precise in genetic variability evaluation then conventional methods. Variability of 96 wheat cultivars and lines was analyzed using four microsatellite markers (Gwm11, Gwm428, Psp3200, Psp3071). The markers were chosen according to their potential association with important agronomical traits indicates in the literature. Total of 31 alleles were detected with maximum number of alleles (11) in Xgwm11 locus. The highest polymorphism information content (PIC) value (0.831) was found in the locus Xpsp3071. The genotypes were grouped into three subpopulations based on their similarity in the analyzed loci. The results have indicated wide genetic variability of the studied material and possibility of its application in further breeding process after validation of marker-trait association.
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