Implementation of polymerase chain reaction (PCR) and real-time PCR in quick identification of bovine herpesvirus 1 | Primena lančane reakcije polimeraze (PCR) i metode Real-time PCR u brzoj identifikaciji goveđeg herpesvirusa 1
2010
Milić, N., Fakultet veterinarske medicine, Beograd (Serbia) | Nišavić, J., Fakultet veterinarske medicine, Beograd (Serbia) | Ašanin, R., Fakultet veterinarske medicine, Beograd (Serbia) | Knežević, A., Medicinski fakultet, Beograd (Serbia) | Ašanin, J., Naučni institut za veterinarstvo Srbije, Beograd (Serbia) | Vidanović, D., Veterinarski specijalistički institut Kraljevo, Kraljevo (Serbia) | Šekler, M., Veterinarski specijalistički institut Kraljevo, Kraljevo (Serbia)
Examinations were performed on 65 samples of nasal smeas taken from calves and young cows with clinical symptoms of respiratory infection to determine the presence of the bovine herpesvirus 1 using parallel implementation of molecular and standard methods of virological diagnostics. The appearance of a cytopathogenic effect (CPE) was not established in innoculated cell lines 24h, 48h and 72h following innoculation, or after two successive pasages of the examined material sample through these cell lines. The application of polymerase chain reaction (PCR) using a primer for glucoprotein B and thymidine – kinasis, established the presence of bovine herpesvirus 1 nucleic acid in one sample of a bovine nasal smear, while the presence of this virus was established in three samples in an examination of the nasal smear samples using the Real-Time PCR method.
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