Reliability of ELISA method in detection of chloramphenicol in meat of rainbow trout | Pouzdanost ELISA metode u detekciji hloramfenikola u mesu kalifornijske pastrmke
2011
Tanković, S., Ured za veterinarstvo Bosne and Hercegovine, Sarajevo (Bosnia and Herzegovina) | Nedić, D., Ured za veterinarstvo Bosne and Hercegovine, Sarajevo (Bosnia and Herzegovina) | Smajlović, M., Veterinarski fakultet, Sarajevo (Bosnia and Herzegovina) | Članjak, E., Veterinarski fakultet, Sarajevo (Bosnia and Herzegovina) | Bilandžić, N., Hrvatski veterinarski institut u Zagrebu, Zagreb (Croatia) | Janković, S., Institut za higijenu i tehnologiju mesa, Beograd (Serbia) | Vesković-Moračanin, S., Institut za higijenu i tehnologiju mesa, Beograd (Srbija) | Cerkvenik-Flajs, V., Reziduum residue consulting, Ljubljana (Slovenia)
In order to determine the current situation in terms of readiness of veterinary service to respond to the incident situation, we decided to do What if studies, i.e. simulation of using banned substance – chloramphenicol, on the pond where rainbow trout is breed, intended for further sale and-or for processing market. Usage of chloramphenicol on the animals used for human consumption in Bosnia and Herzegovina and European Union is legally forbidden. In spite of mentioned ban, chloramphenicol is still a drug of choose in some countries, because it is relatively cheep and available on the market, choice which presumes its illegal usage. During the implementation of the experiment, and during the analytical processing of collected samples, we used the available screening methods (ELISA) in the currently available laboratory capacities in Bosnia and Herzegovina. Due to the lack of adequate laboratory equipment to perform conformational methods (LC-MS/MS) in Bosnia and Herzegovina, we have performed them outside our country.
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